Oxidative Degradation of Benzo[a]pyrene by the Ligninolytic Fungi

نویسندگان

  • Tony HADIBARATA
  • T. HADIBARATA
چکیده

A maximal degradation rate (76%) was obtained when Polyporus sp. S133 was cultured with 0.05% of tween 80, as compared to just 35% degradation in non-supplemented cultures. When fungus Polyporus sp. S133 was grown in presence of shitake’s nutrient, the degradation rate increased up to 69% as compared to just 56 and 40% degradation in glucose and polypeptone supplemented cultures. One metabolite was identified as benzo[a]pyrene-6,12quinone, using UV-VIS chromatography, gas chromatography-mass spectrometry (GC-MS) and thin layer chromatography (TLC). Fungus Polyporus sp. S133 initiates benzo[a]pyrene degradation via deoxygenation at the C-6 and C12 ring positions to form quinine compound. For the first time, metabolism of benzo[a]pyrene in soil by Polyporus sp. S133 was investigated.

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تاریخ انتشار 2009